Journal: Journal of Pharmaceutical Analysis

Article Title: Quantitation of DNA by nuclease P1 digestion and UPLC-MS/MS to assess binding efficiency of pyrrolobenzodiazepine

doi: 10.1016/j.jpha.2020.05.011

Figure Lengend Snippet: Representative chromatograms in UPLC-MSMS analysis: (A) 20 ng/mL equimolar mixture of the 4 deoxyribonucleotides; (B) 20 ng/mL CT DNA after nuclease P1 digestion; (C) 100 μg/mL CT DNA spiked with 100 μg/mL ribonucleotide equimolar mixture, after nuclease P1 digestion (200-fold diluted before injection); (D) DNA isolated from mouse tumor, after nuclease P1 digestion (200-fold diluted before injection); (E) 20 ng/mL CT DNA after DNase I digestion.

Article Snippet: Calf thymus (CT) DNA was purchased from Rockland Immunochemicals (Pottstown, PA, USA).

Techniques: Injection, Isolation

Journal: Journal of Pharmaceutical Analysis

Article Title: Quantitation of DNA by nuclease P1 digestion and UPLC-MS/MS to assess binding efficiency of pyrrolobenzodiazepine

doi: 10.1016/j.jpha.2020.05.011

Figure Lengend Snippet: Method validation for LC-MS/MS analysis of DNA.

Article Snippet: Calf thymus (CT) DNA was purchased from Rockland Immunochemicals (Pottstown, PA, USA).

Techniques: Biomarker Discovery, Concentration Assay

Journal: Journal of Pharmaceutical Analysis

Article Title: Quantitation of DNA by nuclease P1 digestion and UPLC-MS/MS to assess binding efficiency of pyrrolobenzodiazepine

doi: 10.1016/j.jpha.2020.05.011

Figure Lengend Snippet: Comparative quantitation of DNA by UV and LC-MS/MS of dAMP after DNA hydrolytic digestion.

Article Snippet: Calf thymus (CT) DNA was purchased from Rockland Immunochemicals (Pottstown, PA, USA).

Techniques: Quantitation Assay, Concentration Assay

Journal: Genes and Environment

Article Title: Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

doi: 10.1186/s41021-022-00236-3

Figure Lengend Snippet: DNA synthesis and selective nucleotide incorporation opposite urea (Ua) by DNA polymerase α (panel A), DNA polymerase β (panel B) and Kf exo − (panels C and D). DNA polymerase α (100 μU), DNA polymerase β (250 μU) and Kf exo − (75 μU) ware incubated with templates containing G (lanes 1–5) or Ua (lanes 6–10) and 100 μM of each of the four dNTPs (lanes 5 and 10) or 100 μM of a single dNTP (N = C, G, A, or T) (lanes 1–4 and 6–9). Lane M contained no enzyme and are negative controls. The background darkness of panel C has been adjusted in panel D, and only the part of the gel around the 30-mer full length products is shown

Article Snippet: Calf thymus DNA polymerase α and human DNA polymerase β were purchased from Chimerx (Milwaukee, USA).

Techniques: DNA Synthesis, Incubation

Journal: Genes and Environment

Article Title: Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

doi: 10.1186/s41021-022-00236-3

Figure Lengend Snippet: DNA synthesis and selective nucleotide incorporation opposite urea (Ua) by DNA polymerase δ (panels A and B) and DNA polymerase ε (panel C). DNA polymerase δ (154 ng) and DNA polymerase ε (50 μU) ware incubated with templates containing G (lanes 1–5) or Ua (lanes 6–10) and 100 μM of each of the four dNTPs (lanes 5 and 10) or 100 μM of a single dNTP (N = C, G, A, or T) (lanes 1–4 and 6–9). Lane M contained no enzyme and are negative controls. The background darkness of panel A has been adjusted in panel B, and only the part of the gel around the 30-mer full length products is shown

Article Snippet: Calf thymus DNA polymerase α and human DNA polymerase β were purchased from Chimerx (Milwaukee, USA).

Techniques: DNA Synthesis, Incubation

Journal: Genes and Environment

Article Title: Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

doi: 10.1186/s41021-022-00236-3

Figure Lengend Snippet: DNA synthesis and selective nucleotide incorporation opposite urea (Ua) by DNA polymerase ζ (panel A), DNA polymerase η (panel B) and Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) (panel C). DNA polymerase ζ (1.7 ng), DNA polymerase η (0.4 ng) and Dpo4 (0.4 ng) ware incubated with templates containing G (lanes 1–5) or Ua (lanes 6–10) and 100 μM of each of the four dNTPs (lanes 5 and 10) or 100 μM of a single dNTP (N = C, G, A, or T) (lanes 1–4 and 6–9). Lane M contained no enzyme and are negative controls

Article Snippet: Calf thymus DNA polymerase α and human DNA polymerase β were purchased from Chimerx (Milwaukee, USA).

Techniques: DNA Synthesis, Incubation